ISOLASI DAN KARAKTERISASI RHIZOBAKTERI YANG BERPOTENSI SEBAGAI AGEN PEMACU PERTUMBUHAN TANAMAN

Eko Hary Pudjiwati; Siti Zahara; Dewi Sartika

doi:10.35334/borneo_saintek.v2i2.1084

ISOLASI DAN KARAKTERISASI RHIZOBAKTERI YANG BERPOTENSI SEBAGAI AGEN PEMACU PERTUMBUHAN TANAMAN

Eko Hary Pudjiwati, Siti Zahara, Dewi Sartika

Abstract

Penelitian bertujuan untuk mendapatkan isolat rizobakteri yang memiliki aktifitas melarutkan fosfat, memproduksi enzim protease, dan memiliki aktifitas anti-fungal terhadap cendawan fitopatogen Fusarium oxysporum secara in vitro. Isolasi rizobakteri dilakukan di Laboratorium Perlindungan Tanaman Fakultas Pertanian Universitas Borneo Tarakan dari sampel tanah yang diambil dari daerah di sekitar Kota Tarakan, Kalimantan Utara dengan media Nutrient Agar (NA). Isolat bakteri yang diperoleh diuji keamanannya sebagai agens hayati dengan cara menumbuhkan benih timun yang telah direndam dalam suspensi bakteri. Bakteri yang aman (tidak berpotensi sebagai fitopatogen) kemudian dikarakterisasi fenotipnya dan sifat fisiologisnya. Sifat fisiologis yang diamati adalah kemampuan dalam menghasilkan enzim protease (pada media Skim Milk Agar, Merck, Germany) dan kemampuannya dalam melarutkan fosfat (pada media Pikovskaya Agar, HiMedia, India). Selanjutnya juga diuji kemampuannya dalam menghambat pertumbuhan F. oxysporum secara in vitro. Hasil penelitian meunjukkan bahwa diperoleh 35 isolat rhizobakteri yang non patogenik, 23 isolat rhizobakteri yang memiliki aktivitas proteolitik, 2 isolat rhizobakteri yang mampu melarutkan Fosfat dan tidak ada isolat rhizobakteri yang memiliki kemampuan antifungal.

Keywords

Isolat; Rhizobakteri; Agen; Pemacu Pertumbuhan Tanaman; Karakterisasi

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DOI: https://doi.org/10.35334/borneo_saintek.v2i2.1084

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