EKSTRAKSI BIJI PEPAYA (Carica papaya) SEBAGAI BAHAN AKTIF ANTIBAKTERIAL PADA LARVA UDANG WINDU (Penaeus monodon) SECARA IN VITRO DAN IN VIVO

Jimmy Cahyadi, Laylan Sovina

Abstract


This research was the administration of papaya seed extract to black tiger prawn which were challenged with Vibrio harveyii infection in vivo and in vitro to determine the effectiveness of papaya seed extract in inhibiting vibrio harveyii. This research was conducted in the laboratory of the Faculty of Fisheries and Marine Sciences, University of Borneo Tarakan in vitro and in vivo. The data obtained were analyzed descriptively and statistically. Based on observations and research it is known that papaya seed extract (Carica papaya) in vitro contains active antibacterial ingredients such as saponins, flavonoids which can inhibit Vibrio in breeding so that it has an impact on the weakness of Vibrio harveyii in disturbing and inhibiting the growth of tiger shrimp larvae during independent research. in vivo. The survival rate of black tiger prawn larvae from the total of each treatment which was maintained for 12 days of treatment showed that the highest value was achieved in treatment P3 of 90% and the lowest was obtained in the negative control treatment of 5%, while in treatment P1 (positive control) of 95 %, P4 was 78% and P2 treatment was 65%.

Keywords


Black Tiger Prawn larvae, Vibrio harveyi, Carica papaya

Full Text:

PDF

References


 Ariani, N., & Niah, R. (2018). Pengujian Daya Hambat Ekstrak Kulit Buah Pisang Kepok

(Musa paradisiaca forma typica) Terhadap Shigella dysenteriae Dan Salmonella typhi.

Jurnal Ilmiah Ibnu Sina, 3(2), 312–317.

 [AOAC] Association of Official Analytical Chemist. 2005. The Association of official analytical chemist.16th edition. Virginia (US):AOAC.Inc Arlington

 Behera, S., Ganthy, S., Ahmad, F., Santra, S., & Banerjee, S., 2012. Uv-Visible Spectrophotometric Method Development and Validation of Assay of Paracetamol Tablet Formulation. Journal of Analytical & Bioanalytical Techniques, 3(6). https://doi.org/10.4172/2155- 9872.1000151

 Bangham, A.D., and Horne R.W. 2006. Action of saponins on biological cell membrane.

Journal Nature 196: 952-953.

 Cushnie dkk., 2014. Alkaloid an overview of antibacterial , antibiotic-enancing and

antivirulent activity. International Journal of Antimicrobial Agent. 44: 377- 386.

 Cahyadi J,. 2021. Manajemen Perikanan Budidaya Air Payau dan Laut (prinsip dan praktik). Syiah Kuala Universoty Press. ISBN : 978-623-264-325-3. 101 hlm.

 Cahyadi, J., Satriani GI., Gusman E., Sabri. 2019. Ekstrak Buah Mangrove (Sonnetaria

alba) Pada Artemia salina Dalam Menghambat Infeksi Vibrio harveyi Terhadap Sintasan

Benur Udang Windu (Peneaus monodon) Secara In Vivo. Jurnal Harpodon Borneo. ISSN:

-121X Vol 12 Nomor 1 April 2019. Universitas Borneo Tarakan.

 Cahyadi J., Satriani, G.I., Gusman E., Weliyadi E., 2018. Buku Parasit dan Penyakit Ikan “Parasite and Fish Diseaseâ€. Myria Publisher. ISBN. 978-602-53663-0-7. www.myriabook.com

 Dhiah, Novalina, Sugiyarto dan Ari Susilowati, 2013, Aktivitas Antibakteri Ekstrak Carica pubescens dari Dataran Tinggi Dieng terhadap Bakteri Penyebab Diare, Universitas Sebelas Maret : Surakarta.

 Ergina, Nuryanti, S., & Pursitasari, I., 2014. Uji Kualitatif Senyawa Metabolit Sekunder pada Biji Palado (Agave angustifolia) yang DiEkstraksi dengan Pelarut Air dan Etanol. Jurnal Akademika Kimia, 3(3): 165–172

 Effendi H. 2003. Telaah Kualitas Air : Bagi Pengelolaan Sumberdaya dan Lingkungan Perairan. Jurusan managemen Sumberdaya Perairan. FPIK. IPB. Bogor. 258 hal.

 Febrianti, Dwi Rizki. 2019. Aktivitas Antibakteri Minyak Atsiri Kulit Jeruk Siam Banjar

(Citrus reticulata) Terhadap Pertumbuhan Pseudomonas aeruginosa. Jurnal

Pharmascience. Vol. 06 , No.01, Februari 2019, hal: 10 – 17.

 Farida, R., Dewa, M., Titis, N. dan Endrawati, T. 2010. Manfaat Sirih Merah (Piper

crocatum) Sebagai Agen Antibakterial terhadap Bakteri Gram Positif dan Gram Negatif.

Jurnal Kedokteran dan Kesehatan Indonesia, I (7): Hal. 10-25.

 Gazali, M., dan Safutra, E. 2016. Skrenning Potensi Antibakteri Ekstrak Padina Australis

Hauck Terhhadap Bakteri Vibrio harveyii. Jurnal Perikanan Tropis 2 (2), ISSN: 2355-

 Hadioetomo, 1990. “Mikrobiologi Dasar Dalam Praktekâ€. Penerbit Gramedia 161 halaman : Jakarta

 Mulyono, Lienny Meriyuki. 2013. Aktivitas Antibakteri Ekstrak Etanol Biji Buah Pepaya

(Carica papaya L) terhadap Escherichia coli dan Staphylococcus aureus. Jurnal Ilmiah

Mahasiswa Universitas Surabaya vol.2 no.2.

 Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols Dj, McLaughlin JL. 1982. Brine shrimp: A convenient general bioassay for active plant constituents. Planta medica. (45): 31-34

 Nuria, MC., Faizatun, A., Sumantri. 2009. Pengujian Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha curcas L.) Terhadap Bakteri Staphylococcus aureus

Atcc 25923, Escherichia coli Atcc 25922, Dan Salmonella thypi Atcc 1408. Jurnal Ilmuilmu Pertanian, 5:26-37.

 Paramesti, N. N. 2014. Efektivitas Ekstrak Biji buah pepaya (Carica papaya L.) Sebagai

Antibakteri Terhadap Bakteri Escherichia coli. Skripsi. Program Studi Pendidikan

Dokter. Universitas Islam Negeri Syarif Hidayatullah. Jakarta

 Pelczar, M. J., and Chan, E. C. S., 2007. Elements of Microbiology. Mc Graw Hill Book Company: New York. Diterjemahkan oleh Hadioetomo, R. S., Penerbit Universitas Indonesia. Jakarta

 Roni, A., Maesaroh, Lia, Marliani. 2018. Aktivitas Antibakteri Biji, Kulit, dan Daun

Pepaya (Carica papaya L.) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Ilmiah Farmasi. 6 (1): 29-33. Sekolah Tinggi Farmasi Bandung.

 Robinson T. 1995. Kandungan Organik Tumbuhan Tinggi. Penerjemah: K.

Padmawinata. Edisi IV. Bandung: ITB Press

 Sari, A.P., 2019. Sensitivitas Aeromonas hydropillar Terhadap Ekstrak Daun Jambu biji

(Psidium guajava) Secara In-vitro dan Lc50 pada Ikan Nila Merah (Oreochromis sp).

Skripsi Sarjana. Program Studi Akuakultur. Fakultas Perikanan dan Ilmu Kelautan.

Universitas Borneo Tarakan. Tarakan

 Sukadana, I. M, Santi, S.R, & Juliarti N.K. 2008. Aktivitas Antibakteri Senyawa Golongan Triterpenoid dari Biji Pepaya (Carica papaya L.). Jurnal Kimia. 2 (1), 15-18

 Sabir, A. 2005. Aktivitas Antibakteri Flavonoid Propolisis Trigona sp. Terhadap Bakteri

Streptococcus mutans (In-Vitro). Jurnal Kedokteran Gigi. 38 (3):135.

 Tandi, J., 2017. Effect of Ethanol Extract Gendola Leaf Leaf (Basella alba L.) on Decreasing Blood Glucose Condition and Histopatology Pankreas White Rats (Rattus norvegicus) Indicated Streptozotocin. JIMR - Journal of Islamic Medicine Research, 1(2): 15–25.

 Wijayanti, R dan Febrinasari N. 2017. Karakterisasi Ekstrak Biji buah pepaya (Carica pubescens) serta Pengujian Antibakteri terhadap Enterophatogenic Escherichia coli

(EPEC) Penyebab Diare pada Mencit Jantan. CERATA Jurnal Ilmu Farmasi. Issue 1, Vol.

Hal. 1-13. Universitas Islam Sultan Agung. Semarang. Publisher: Stikes

Muhammadiyah Klaten.

 Yanti, Y. N. dan Mitika, S. 2017. Pengujian Efektivitas Antibakteri Ekstrak Etanol Daun

Sambiloto (Andrographis paniculata Nees) Terhadap Bakteri Staphylococus aureus.

Jurnal Ilmiah Ibnu Sina. 2(1): 158-168. Akademi Farmasi Yayasan Al-Fatah. Bengkulu.

 Zukhri, Saifudin. (2015). Efektifitas Antibakteri Ekstrak Etanol Biji buah pepaya (Carica

papaya L) Terhadap Bakteri Escherichia coli, Motorik, Vol.10 No.20




DOI: https://doi.org/10.35334/borneo_saintek.v5i1.4136

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Jurnal Borneo Saintek

slot dana

PODOMORO138 SEDAYU138 JALURDEWA MERAHBET